Estudios de parentesco mediante marcadores del ADN: Experiencia en resolución de casos en los últimos seis años / Kinship determination using DNA markers
Rev. méd. Chile
;
136(2): 193-200, feb. 2008. tab
Artigo
em Espanhol
| LILACS
| ID: lil-483239
ABSTRACT
Autosomal and Y chromosome short tandem repeats (STRs) and mitochondrial DNA polymorphisms are the most commonly used molecular tools for determination of kinship. Aim:
To report a revision of 1,120 kinship cases (paternity and others) analyzed in our laboratory. Material andmethods:
Revision of all kinship cases analyzed between years 2001-2006. Autosomal and Y chromosome STRs and mitochondrial DNA polymorphisms were analyzed in DNA extracted from blood samples.Results:
Paternity was excluded in 27.2 percent of cases. This figure did not change significantly along years. Most paternity exclusions were confirmed by the discordance in 5 genetic markers (30.5 percent), followed by exclusion of 4 and 6 genetic markers (20.3 and 20 percent respectively). Two studied cases were paternal and maternal exclusions, corresponding to a change of children between two families. In one case, the paternal line was assessed through Y chromosome markers, studying 16 STRs of this chromosome, positively confirming this paternal relationship. Another case was analyzed for maternal line using mitochondrial DNA analysis. In six cases, a genetic marker with a paternal-sibling mutation, was observed. The criteria for the determination of mutation was the finding of only one discordant marker between at ¡east thirteen markers analyzed in each case. Also, an increase or decrease in one unit repeated in tandem (tetranucleotide) between the alleged father and the son was also required. One subject had a double mutation. In this case, paternity was confirmed analyzing thirteen autosomic STRs and five Y-STRs.Conclusions:
The authors have acquired great expertise in kinship analysis and had established criteria to solve complex kinship cases.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Paternidade
/
Impressões Digitais de DNA
/
Repetições de Microssatélites
/
Cromossomos Humanos Y
Tipo de estudo:
Estudo observacional
Limite:
Criança
/
Feminino
/
Humanos
/
Masculino
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Genética y Tecnología Ltda/CL
/
Universidad de Chile/CL
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