Use of chromosome microdissection in fish molecular cytogenetics
Genet. mol. biol
;
31(1,suppl): 279-283, 2008. ilus
Artigo
em Inglês
| LILACS
| ID: lil-484601
ABSTRACT
Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Cromossomos Sexuais
/
Microdissecção
/
Peixes
Limite:
Animais
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Federação Russa
Instituição/País de afiliação:
Siberian Branch of the Russian Academy of Sciences/RU
/
Universidade de São Paulo/BR
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