Isolation and molecular identification of Leishmania chagasi from a bat (Carollia perspicillata) in northeastern Venezuela
Mem. Inst. Oswaldo Cruz
;
103(4): 412-414, June 2008. ilus
Artigo
em Inglês
| LILACS
| ID: lil-486869
ABSTRACT
This report describes the isolation of a Leishmania chagasi strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inoculated into the footpads of Balb/c mice, which subsequently developed a typical nodular leishmanial lesion; the parasites were confirmed as Leishmania by smear and histopathology. Molecular characterization of the parasites was performed by polymerase chain reaction with species-specific primers, kDNA restriction pattern following Hae III endonuclease digestion and dot blot hybridization using a kDNA probe. This report demonstrates that bats can be hosts for L. chagasi species and suggests the need for studies to determine whether they may be involved in foci of visceral leishmaniasis.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Reservatórios de Doenças
/
Quirópteros
/
Leishmania infantum
/
DNA de Cinetoplasto
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Venezuela
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Venezuela
Instituição/País de afiliação:
Geoclean C.A/VE
/
Universidad Central de Venezuela/VE
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