Plant regeneration of natural tetraploid Trifolium Hum pratense L
Biol. Res
;
41(1): 25-31, 2008. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-490629
ABSTRACT
The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm-3 BAP and 1 mg dm-3 NAA. 94.4 percent of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm-3 NAA. In vitro organogénesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Reguladores de Crescimento de Plantas
/
Regeneração
/
Meios de Cultura
/
Trifolium
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Turquia
Instituição/País de afiliação:
Ankara University/TR
/
Zonguldak Karaelmas University/TR
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