Caracterización molecular en aislados chilenos de Streptococcus agalactiae / Molecular characterization of Chilean isolates of streptococcus agalactiae
Rev. méd. Chile
;
136(5): 606-612, mayo 2008. ilus
Artigo
em Espanhol
| LILACS
| ID: lil-490698
ABSTRACT
Streptococcus agalactiae is the main causing organism of invasive infections such as sepsis and meningitis in the newborn. Aim:
To perform a genotype characterization of Streptococcus agalactiae strains coming form invasive infections of newborns and colonized pregnant women. Material andmethods:
A group of 58 strains not related epidemiologically isolated from colonized pregnant women and invasive infections in newborns, were studied. Pulsed field electrophoresis (PFGE) and polymerase chain reaction amplification of hylB and IS 1548 genes, as possible virulence markers, were performed.Results:
Among the studied strains, 37 genetic subtypes were observed. There were nine groups of identical PFGE patterns. Three corresponded to serotype la and six to serotype III. An erythromycin and clindamycin resistant clone was identified in three colonized women and a newborn with sepsis, which were not epidemiologically related. The hylB gene was equally present in cases of neonatal meningitis or colonized pregnant women.Conclusions:
There was a great degree of polymorphism among the studied strains. The ample presence of hylB gene and the absence of the insertion element IS1548 in the hylB gene in invasive and colonizing strains, indicates that both groups of strains are potentially pathogenic.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Infecções Estreptocócicas
/
Streptococcus agalactiae
/
Genes Bacterianos
Limite:
Feminino
/
Humanos
/
Recém-Nascido
/
Gravidez
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Instituto de Salud Pública de Chile/CL
/
Universidad de Chile/CL
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