Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
Biocell
;
31(1): 51-59, abr. 2007. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-491537
ABSTRACT
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 microM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of Development. The incubation with 50 microM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p < 0.001). Twenty-five microM H2O2 produced inhibition of blastocyst formation (p < 0.001) and 10 microM H2O2 significantly decreased the percentages of expanded and hatchedblastocysts, which resulted morphologically altered (p < 0.05 and p < 0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 microM H2O2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Blastocisto
/
Fase de Clivagem do Zigoto
/
Espécies Reativas de Oxigênio
/
Peróxido de Hidrogênio
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2007
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad de Chile/CL
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