Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia
Mem. Inst. Oswaldo Cruz
;
103(5): 493-496, Aug. 2008. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-491980
ABSTRACT
We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3 gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 percent). The Pvmsp-3 gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plasmodium vivax
/
Polimorfismo Genético
/
DNA de Protozoário
Limite:
Animais
/
Humanos
País/Região como assunto:
América do Sul
/
Colômbia
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2008
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Colômbia
Instituição/País de afiliação:
Instituto Nacional de Salud/CO
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