The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom
J. venom. anim. toxins incl. trop. dis
;
14(3): 423-434, 2008. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-492207
ABSTRACT
The present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n equal 24 each) Group S (0.9 percent saline solution); Group V (venom) and Group VLLL (venom plus low-level laser). These categories were subdivided into four additional groups (n equal 6) based on the euthanasia timing (3 hours, 24 hours, 3 days and 7 days). The groups V and VLLL were inoculated with 100 miL of concentrated venom (40 mig/mL) in the gastrocnemius muscle. The muscle was irradiated using a gallium-aluminum-arsenide laser (GaAlAs) at 35 mW power and 4 J/cm² energy density for 3 hours, 24 hours, 3 days or 7 days after venom inoculation. To evaluate the myotoxic activity of the venom, CK activity was measured and the muscle was histologically analyzed. The low-level laser reduced venom-induced CK activity in the groups euthanized at 3 hours, 24 hours and 3 days (p smaller that 0.0001). Histological analysis revealed that low-level laser reduced neutrophilic inflammation as well as myofibrillar edema, hemorrhage and myonecrosis following B. neuwiedi envenomation. These results suggest that low-level laser can be useful as an adjunct therapy following B. neuwiedi envenomation.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ratos Wistar
/
Bothrops
/
Venenos de Crotalídeos
/
Terapia com Luz de Baixa Intensidade
Limite:
Animais
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Nove de Julho University/BR
/
University for the Development of the State and of the Region of Pantanal/BR
/
University of São Paulo/BR
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