Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
Braz. j. infect. dis
;
12(4): 316-320, Aug. 2008. tab
Artigo
em Inglês
| LILACS
| ID: lil-496771
ABSTRACT
The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71 percent of the isolates. All methods gave 100 percent sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100 percent, while the oxacillin disk gave a specificity of 91 percent and agar dilution oxacillin gave a specificity of 88 percent. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillin-resistance in staphylococci.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Staphylococcus
/
Proteínas de Bactérias
/
Resistência a Meticilina
/
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
/
Antibacterianos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Health Sciences of Porto Alegre/BR
/
Weinmann Laboratory/BR
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