Membrane damage of bacteria by silanols treatment
Electron. j. biotechnol
;
10(2): 252-259, Apr. 15, 2007. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-499176
ABSTRACT
Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were treated by silanols at concentration of less than 0.2 wt percent for one hour. Membrane damage of the bacteria by the silanol treatment was clearly observed by transmission electron microscopy. Separation of the cytoplasmic membrane from the outer membrane for E. coli and disorganized cytoplasmic membrane of the Gram-positive bacteria were observed when compared to the control. Fluorescent dyes, green-fluorescent nucleic acid stain (Syto 9) and the red-fluorescent nucleic acid stain (Propidium iodide), were used to monitor membrane damage of the bacteria by Confocal microscopy and Spectrophotometer. A reduction of the green fluorescent emission was detected for silanol treated bacteria indicating membrane damage of the bacteria and supporting the hypothesis that their viability loss may be due to their membrane damage analogus to alcohols.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Silanos
/
Bactérias
/
Membrana Celular
/
Interações Hidrofóbicas e Hidrofílicas
/
Antibacterianos
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2007
Tipo de documento:
Artigo
País de afiliação:
Estados Unidos
Instituição/País de afiliação:
University of Florida/US
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