Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
Genet. mol. biol
;
32(1): 155-158, 2009. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-505775
ABSTRACT
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Dano ao DNA
/
Ensaio Cometa
/
Eritrócitos
/
Peixes
Limite:
Animais
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual Paulista Júlio de Mesquita Filho/BR
/
Universidade Federal do Pará/BR
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