Phenotypic detection and occurrence of extended-spectrum beta-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli at a tertiary Hospital in Trinidad & Tobago
Braz. j. infect. dis
;
12(6): 516-520, Dec. 2008. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-507453
ABSTRACT
The incidence and distribution of ESBL producing microorganisms such as E. coli and K. pneumoniae have been demonstrated and varies in different health care facilities and as well as other countries This study was carried out to determine the frequency of occurrence and the antimicrobial susceptibility pattern of ESBL producing E. coli and K. pneumoniae species from clinical isolates at a tertiary hospital in Trinidad & Tobago. Standard microbiological procedures and automated MicroScan System was used to identify, screen for putative ESBL production and determine antimicrobial susceptibility of 1,118 clinical isolates of Enterobacteriaceae species at the microbiology laboratory of the Eric Williams Medical Science Complex, Trinidad & Tobago over a 36 months period. All ESBL producing isolates flagged by the automated system were further confirmed by E-test method. The E-test confirmed a 15.2 percent ESBL rate among the K. pneumoniae isolates and 9.3 percent among the E. coli isolates. There was also a 1.8 percent rate of ESBL production in K. pneumoniae and 0.2 percent in E. coli isolates from specimens received from community health facilities into the laboratory. Isolates recovered from the intensive care unit of the hospital had 2.1 percent E. coli and 8.2 percent K. pneumoniae ESBL producers. Although all ESBL positive isolates were completely susceptible to imipenem and meropenem; and all positive K. pneumoniae isolates were susceptible to amikacin, there was a low susceptibility of ESBL positive E. coli to the aminoglycosides. However, susceptibility of these ESBL producing isolates to the fluoroquinolones varied. There is a high rate of ESBL production among isolates of E. coli and K. pneumoniae at this hospital that is linked to the extensive inappropriate use of third generation cephalosporins in the country. Further molecular studies are needed to characterize the types of these ESBL prevailing in the country.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Beta-Lactamases
/
Escherichia coli
/
Klebsiella pneumoniae
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
/
Fatores de risco
Limite:
Humanos
País/Região como assunto:
Caribe Inglês
/
Trinidad e Tobago
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Trinidad e Tobago
Instituição/País de afiliação:
The University of the West Indies/TT
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