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Screening and enzymatic study of a composite microbial system FH3
Zhang, Yi-Min; Lu, Xue-Bin; Dan, Han-Bin; Sun, Ya-Kai.
  • Zhang, Yi-Min; Tianjin University. School of Chemical Engineering and Technology. Key Laboratory of Green Chemical Technology. CN
  • Lu, Xue-Bin; Tianjin University. School of Chemical Engineering and Technology. Key Laboratory of Green Chemical Technology. CN
  • Dan, Han-Bin; City University of New York. Lehman College. Department of Biological Sciences. US
  • Sun, Ya-Kai; Tianjin University. School of Chemical Engineering and Technology. Key Laboratory of Green Chemical Technology. CN
Braz. arch. biol. technol ; 52(1): 35-43, Jan.-Feb. 2009. graf, tab
Artigo em Inglês | LILACS | ID: lil-511674
ABSTRACT
Five strains of fungi and 18 strains of bacteria were isolated from the soil and horse manure with cellulose as the sole carbon source. Among them, two fungal, F9 and F13, and two bacterial, B16 and B21, showed the highest filter paper activities, which were 7.79 U g-1, 9.84 U g-1, 7.34 U g-1 and 9.68 U g-1, respectively. Four microbial systems, designed as FH1 (F13+B21), FH2 (F13+B16+B21), FH3 (F9+B16+B21) and FH4 (F13+F9+B16+B21) were developed. The fermentation studies showed that the filter paper activity of the composite microbial system FH3 was higher than the others, which was 21.34 U g-1. The medium with bran and filter paper as the carbon source and peptone as nitrogen source was optimal and the maximum cellulase activity was reached at 30~35ºC and pH 6.0~6.5 when FH3 was incubated for 48 h. The enzymatic reaction conditions were estimated at 45~55 ºC, pH 4.5~5.5 and the thermal stability temperature was up to 60 ºC.

Texto completo: DisponíveL Índice: LILACS (Américas) Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2009 Tipo de documento: Artigo País de afiliação: China / Estados Unidos Instituição/País de afiliação: City University of New York/US / Tianjin University/CN

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Texto completo: DisponíveL Índice: LILACS (Américas) Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2009 Tipo de documento: Artigo País de afiliação: China / Estados Unidos Instituição/País de afiliação: City University of New York/US / Tianjin University/CN