Screening and enzymatic study of a composite microbial system FH3
Braz. arch. biol. technol
;
52(1): 35-43, Jan.-Feb. 2009. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-511674
ABSTRACT
Five strains of fungi and 18 strains of bacteria were isolated from the soil and horse manure with cellulose as the sole carbon source. Among them, two fungal, F9 and F13, and two bacterial, B16 and B21, showed the highest filter paper activities, which were 7.79 U g-1, 9.84 U g-1, 7.34 U g-1 and 9.68 U g-1, respectively. Four microbial systems, designed as FH1 (F13+B21), FH2 (F13+B16+B21), FH3 (F9+B16+B21) and FH4 (F13+F9+B16+B21) were developed. The fermentation studies showed that the filter paper activity of the composite microbial system FH3 was higher than the others, which was 21.34 U g-1. The medium with bran and filter paper as the carbon source and peptone as nitrogen source was optimal and the maximum cellulase activity was reached at 30~35ºC and pH 6.0~6.5 when FH3 was incubated for 48 h. The enzymatic reaction conditions were estimated at 45~55 ºC, pH 4.5~5.5 and the thermal stability temperature was up to 60 ºC.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
China
/
Estados Unidos
Instituição/País de afiliação:
City University of New York/US
/
Tianjin University/CN
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