Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins
Mem. Inst. Oswaldo Cruz
;
104(3): 427-433, May 2009. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-517006
ABSTRACT
Fifty-five clinical and environmental Aspergillus fumigatus isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (I A). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The I A of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin.
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Índice:
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Assunto principal:
Aspergillus fumigatus
/
Variação Genética
/
Meio Ambiente
País/Região como assunto:
América do Sul
/
Argentina
/
Europa
/
México
/
Peru
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2009
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Argentina
/
França
/
México
Instituição/País de afiliação:
Hospital General Dr. Manuel Gea González/MX
/
INEI ANLIS Dr. Carlos G. Malbrán/AR
/
Instituto Politécnico Nacional/MX
/
Lille 2 University/FR
/
Universidad Nacional Autónoma de México/MX
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