Effect of postnatal malnutrition on hyperoxia-induced newborn lung development
Braz. j. med. biol. res
;
42(7): 606-613, July 2009. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-517797
ABSTRACT
Several factors are associated with bronchopulmonary dysplasia. Among them, hyperoxia and lung immaturity are considered to be fundamental; however, the effect of malnutrition is unknown. Our objective was to evaluate the effects of 7 days of postnatal malnutrition and hyperoxia on lung weight, volume, water content, and pulmonary morphometry of premature rabbits. After csection, 28-day-old New Zealand white rabbits were randomized into four groups control diet and room air (CA, N = 17), control diet and ¡Ý95% O2 (CH, N = 17), malnutrition and room air (MA, N = 18), and malnutrition and ¡Ý95% O2 (MH, N = 18). Malnutrition was defined as a 30% reduction of all the nutrients provided in the control diet. Treatments were maintained for 7 days, after which histological and morphometric analyses were conducted. Lung slices were stained with hematoxylin-eosin, modified orcein-resorcin or picrosirius. The results of morphometric analysis indicated that postnatal malnutrition decreased lung weight (CA 0.83 ¡À 0.19; CH 0.96 ¡À 0.28; MA 0.65 ¡À 0.17; MH 0.79 ¡À 0.22 g) and water content, as well as the number of alveoli (CA 12.43 ¡À 3.07; CH 8.85 ¡À 1.46; MA 7.33 ¡À 0.88; MH 6.36 ¡À 1.53 x 10-3/mm) and elastic and collagen fibers. Hyperoxia reduced the number of alveoli and increased septal thickening and the mean linear intercept. The reduction of alveolar number, collagen and elastic fibers was intensified when malnutrition and hyperoxia were associated. These data suggest that dietary restriction enhances the magnitude of hyperoxia-induced alveolar growth arrest and lung parenchymal remodeling. It is interesting to consider the important influence of postnatal nutrition upon lung development and ronchopulmonary dysplasia.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Hiperóxia
/
Desnutrição
/
Pulmão
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Gravidez
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR
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