An in vitro microbial model for producing caries-like lesions on enamel
Braz. j. oral sci
; 6(22): 1392-1396, July-Sept. 2007. tab
Article
em En
| LILACS, BBO
| ID: lil-518165
Biblioteca responsável:
BR28.1
ABSTRACT
This study aimed to develop a low cost in vitro viable microbiological model to produce biofilms to be used in dental researches. Single and multi-species biofilms of S. mutans, S. sobrinus, S. mitis, S. salivarius, S. cricetus and S. sanguinis were grown on bovine enamel slabs during 10 days, in a sterile brain-heart infusion broth, containing 5% sucrose and incubated at 37ºC in an atmosphere of 10% CO2. The slabs were transferred to a fresh medium at every 6, 12 or 24 hours. After the experimental period, enamel volume percent mineral was determined by cross-sectional microhardness. Caries-like lesions were found in all bacterial groups when compared with the control group. No statistical significant differences were found between S. mutans and S. sobrinus with respect of their cariogenicity or among the periods of medium change. However, it was found a statistical significant difference among the cariogenicity of S. salivarius and S. sanguinis (ANOVA followed by Tukey test). This model has successfully developed caries-like lesion on enamel and the medium can be changed at every 24 hours utilizing either S. mutans or S. sobrinus.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Biofilmes
/
Cárie Dentária
/
Esmalte Dentário
Limite:
Animals
Idioma:
En
Revista:
Braz. j. oral sci
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article