Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)
Genet. mol. res. (Online)
; 6(4): 1118-1122, 2007. tab
Article
em En
| LILACS
| ID: lil-520037
Biblioteca responsável:
BR26.1
ABSTRACT
In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, expected heterozygosity was 0.869 and the mean polymorphic information content value was 0.847. Paternity exclusion probabilities over loci were NE-1P = 0.01336 and NE-2P = 0.00135, and combined non-exclusion probability of identity was P(ID) = 3 × 10-8.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Cervos
/
Desequilíbrio de Ligação
/
Repetições de Microssatélites
Limite:
Animals
Idioma:
En
Revista:
Genet. mol. res. (Online)
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA
Ano de publicação:
2007
Tipo de documento:
Article