Candida albicans GRX2, encoding a putative glutaredoxin, is required for virulence in a murine model
Genet. mol. res. (Online)
;
6(4): 1051-1063, 2007. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-520044
ABSTRACT
Resistance of Candida albicans to reactive oxygen species is thought to enhance its virulence in mammalian hosts. Genes such as SOD1, which encodes the anti-oxidant, superoxide dismutase, are known virulence factors. We disrupted the gene GRX2, which encodes a putative glutathione reductase (glutaredoxin) in C. albicans, and we compared the mutant with an sod1 Delta mutant. In vitro, the grx2 Delta strain, but not the sod1 Delta strain, was defective in hypha formation. The grx2 Delta strain, but not sod1 Delta, was significantly more susceptible to killing by neutrophils. When exposed to two compounds that generate reactive oxygen species, both mutants were susceptible to 1 mM menadione, but grx2 Delta null alone was resistant to diamide. Both mutants were attenuated in a murine intravenous challenge model, and a GRX2 reintegrant regained partial virulence. Emphasis on the putative function of products of genes such as SOD1 and GRX2 in resistance to oxidative stress may oversimplify their functions in the virulence process, since the grx2 Delta strain also gave defective hypha formation. Both mutants were sensitive to menadione and were slow to form germ tubes, though growth rates matched controls once the lag phase was passed.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Virulência
/
Candida albicans
/
DNA Fúngico
/
Proteínas
/
Glutationa Redutase
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Genet. mol. res. (Online)
Assunto da revista:
Biologia Molecular
/
Genética
Ano de publicação:
2007
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Índia
Instituição/País de afiliação:
Institute of Medical Sciences/GB
/
Universidade Federal de São Paulo/BR
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