Analysis of genetic diversity among Indian niger [Guizotia abyssinica (L. f.) Cass.] cultivars based on randomly amplified polymorphic DNA markers
Electron. j. biotechnol
;
11(1): 140-144, Jan. 2008. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-522168
ABSTRACT
Randomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 18 cultivars of niger from India. Total genomic DNA was extracted and subjected to RAPD analysis using 80 arbitrary 10-mer primers; 17 primers were selected, which yielded a total of 124 bands, 41.20 percent of them polymorphic. None of the primers produced unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidean Distance matrix which revealed a minimum genetic distance between cultivars JNC-6 and N-48 and a maximum distance between IGP-76 and JN-30. Based on the distance matrix, a cluster analysis was done using a minimum variance algorithm. The dendrogram generated, based on Wards method, grouped 18 niger cultivars into two major clusters. The first cluster consisted of early maturing cultivars (e.g. N-129 and N-134; 80-90 days), and the second of late maturing cultivars (e.g. GA-8 and GA-9; 135-145 days). The present study shows that there is high diversity among the niger cultivars tested and indicates the potential of RAPD markers for identification and maintenance of niger germplasm for crop improvement purposes.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Variação Genética
/
Asteraceae
/
Produção Agrícola
Tipo de estudo:
Ensaio Clínico Controlado
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Índia
/
Coréia do Sul
Instituição/País de afiliação:
Chungbuk National University/KR
/
Indian Institute of Horticultural Research/IN
/
Karnatak University/IN
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