Your browser doesn't support javascript.
loading
Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
Poiatti, Vera A D; Dalmas, Fernando R; Astarita, Leandro V.
  • Poiatti, Vera A D; Pontificia Universidade Católica do Rio Grande do Sul (PUCRS). Faculdade de Biociéncias. Departamento de Biología Celular e Molecular. Laboratorio de Biotecnología Vegetal. Porto Alegre. BR
  • Dalmas, Fernando R; Pontificia Universidade Católica do Rio Grande do Sul (PUCRS). Faculdade de Biociéncias. Departamento de Biología Celular e Molecular. Laboratorio de Biotecnología Vegetal. Porto Alegre. BR
  • Astarita, Leandro V; Pontificia Universidade Católica do Rio Grande do Sul (PUCRS). Faculdade de Biociéncias. Departamento de Biología Celular e Molecular. Laboratorio de Biotecnología Vegetal. Porto Alegre. BR
Biol. Res ; 42(2): 205-215, 2009. ilus, tab
Artigo em Inglês | LILACS | ID: lil-524891
ABSTRACT
The natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. The defense mechanisms present in resistant plants may also be found in susceptible ones. This study attempted to analyze the metabolic alterations in plants of the potato Solanum tuberosum L. cv. Agata that were inoculated with the incompatible plant-pathogenic bacteria X. axonopodis and R. solanacearum, and the compatible bacterium E. carotovora. Levels of total phenolic compounds, including the flavonoid group, and the activities of polyphenol oxidase (PPO) and peroxidase (POX) were evaluated. Bacteria compatibility was evaluated by means of infiltration of tubers. The defense response was evaluated in the leaves of the potato plants. Leaves were inoculated depending on their number and location on the stem. Multiple-leaf inoculation was carried out on basal, intermediate, and apical leaves, and single inoculations on intermediate leaves. Leaves inoculated with X. axonopodis and with R. solanacearum showed hypersensitive responses within 24 hours post-inoculation, whereas leaves inoculated with E. carotovora showed disease symptoms. Therefore, the R. solanacearum isolate used in the experiments did not exhibit virulence to this potato cultivar. Regardless of the bacterial treatments, the basal leaves showed higher PPO and POX activities and lower levels of total phenolic compounds and flavonoids, compared to the apical leaves. However, basal and intermediate leaves inoculated with R. solanacearum and X. axonopodis showed increases in total phenolic compounds and flavonoid levels. In general, multiple-leaf inoculation showed the highest levels of total phenolics and flavonoids, whereas the single inoculations resulted in the highest increase in PPO activity. The POX activity showed no significant difference between single- and multiple-leaf inoculations. Plants inoculated with E. carotovora showed no significant increase ...
Assuntos

Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Doenças das Plantas / Solanum tuberosum / Pectobacterium carotovorum / Ralstonia solanacearum / Xanthomonas axonopodis Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2009 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Pontificia Universidade Católica do Rio Grande do Sul (PUCRS)/BR

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Doenças das Plantas / Solanum tuberosum / Pectobacterium carotovorum / Ralstonia solanacearum / Xanthomonas axonopodis Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2009 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Pontificia Universidade Católica do Rio Grande do Sul (PUCRS)/BR