Use of a saline gradient for the diagnosis of schistosomiasis
Mem. Inst. Oswaldo Cruz
;
104(5): 720-723, Aug. 2009. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-528080
ABSTRACT
The development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of eggs when subjected to a slow continuous flux of 3 percent saline solution through a porous plaque. This influx suspends low-density faecal material, effectively cleaning the sample. The remaining sediment covering the porous plaque surface is then transferred to a glass slide and examined under a bright field microscope. Twelve Kato-Katz slides were used for comparison in the present study. Our results suggest that the saline gradient method detects a signifi-cantly higher number of eggs than the 12 Kato-Katz slides (p < 0.0001). We also found microscopic inspection to be quicker and easier with our newly described method. After cleaning the sample, the obtained sediment can also be conserved in a 10 percent formaldehyde solution and examined for at least 45 days later without statistically significant egg count differences.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Contagem de Ovos de Parasitas
/
Schistosoma mansoni
/
Esquistossomose mansoni
/
Cloreto de Sódio
/
Fezes
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fiocruz/BR
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