Phylogenetic analysis on the soil bacteria distributed in karst forest
Braz. j. microbiol
;
40(4): 827-837, Oct.-Dec. 2009. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-528165
ABSTRACT
Phylogenetic composition of bacterial community in soil of a karst forest was analyzed by culture-independent molecular approach. The bacterial 16S rRNA gene was amplified directly from soil DNA and cloned to generate a library. After screening the clone library by RFLP, 16S rRNA genes of representative clones were sequenced and the bacterial community was analyzed phylogenetically. The 16S rRNA gene inserts of 190 clones randomly selected were analyzed by RFLP and generated 126 different RFLP types. After sequencing, 126 non-chimeric sequences were obtained, generating 113 phylotypes. Phylogenetic analysis revealed that the bacteria distributed in soil of the karst forest included the members assigning into Proteobacteria, Acidobacteria, Planctomycetes, Chloroflexi (Green nonsulfur bacteria), Bacteroidetes, Verrucomicrobia, Nitrospirae, Actinobacteria (High G+C Gram-positive bacteria), Firmicutes (Low G+C Gram-positive bacteria) and candidate divisions (including the SPAM and GN08).
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Filogenia
/
Técnicas In Vitro
/
Polimorfismo de Fragmento de Restrição
/
Sequência de Bases
/
Amplificação de Genes
/
Genes Bacterianos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Yunnan University/CN
Similares
MEDLINE
...
LILACS
LIS