Glass bead transformation method for gram-positive bacteria

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat.

Rattanachaikunsopon, Pongsak; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH
Phumkhachorn, Parichat; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH

Braz. j. microbiol ; 40(4): 923-926, Oct.-Dec. 2009. graf, tab

Artigo em Inglês | LILACS | ID: lil-528176

ABSTRACT

ABSTRACT

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.

Assuntos

Bactérias Gram-Positivas/genética; Genética Microbiana; Protoplastos; Transformação Bacteriana; Vidro/análise; Métodos; Métodos

Glass bead; Gram-positive bacteria; Transformation

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Protoplastos / Transformação Bacteriana / Genética Microbiana / Vidro / Bactérias Gram-Positivas Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2009 Tipo de documento: Artigo País de afiliação: Tailândia Instituição/País de afiliação: Ubon Ratchathani University/TH

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