A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs
Braz. j. microbiol
;
40(4): 988-993, Oct.-Dec. 2009. ilus
Artigo
em Inglês
| LILACS
| ID: lil-528184
ABSTRACT
Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 mL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Vírus de RNA
/
Suínos
/
Técnicas In Vitro
/
Genoma
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Encefalomiocardite
/
Nucleotídeos
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Cuba
Instituição/País de afiliação:
Grupo de Virología/CU
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