Characterization of Leptospira borgpetersenii isolates from field rats (Rattus norvegicus) by 16S rRNA and lipL32 gene sequencing
Braz. j. microbiol
;
41(1): 150-157, Jan.-Mar. 2010. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-531746
ABSTRACT
The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT). Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level. Genomospecies identification was carried out using 16S rRNA and lipL32 gene sequencing. The molecular phylogeny was constructed to find out species segregation. Seroprevalence was about 51.4 percent, and the predominant serovars were Autumnalis, Javanica, Icterohaemorrhagiae and Pomona. Two isolates from the kidneys were identified as serovar Javanica of Serogroup Javanica, and sequence based molecular phylogeny indicated these two isolates were Leptospira borgpetersenii.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Filogenia
/
Sequência de Bases
/
Leptospira interrogans serovar autumnalis
/
Leptospira interrogans serovar icterohaemorrhagiae
/
Leptospira interrogans serovar pomona
/
Leptospirose
Tipo de estudo:
Estudos de avaliação
/
Estudo de prevalência
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Animal Sciences University/IN
/
Bharathidasan University/IN
/
National Centre for Cell Sciences/IN
/
Regional Medical Research Centre/IN
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