Response surface methodology for optimization of production of lovastatin by solid state fermentation
Braz. j. microbiol
;
41(1): 164-172, Jan.-Mar. 2010. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-531748
ABSTRACT
Lovastatin, an inhibitor of HMG-CoA reductase, was produced by solid state fermentation (SSF) using a strain of Aspergillus terreus UV 1718. Different solid substrates and various combinations thereof were evaluated for lovastatin production. Wheat bran supported the maximum production (1458 ± 46 µg g-1 DFM) of lovastatin. Response surface methodology (RSM) was applied to optimize the medium constituents. A 2(4) full-factorial central composite design (CCD) was chosen to explain the combined effects of the four medium constituents, viz. moisture content, particle size of the substrate, di-potassium hydrogen phosphate and trace ion solution concentration. Maximum lovastatin production of 2969 µg g-1 DFM was predicted by the quadratic model which was verified experimentally to be 3004 ± 25 µg g-1 DFM. Further RSM optimized medium supplemented with mycological, peptone supported highest yield of 3723.4±49 µg g-1 DFM. Yield of lovastatin increased 2.6 fold as with compared to un-optimized media.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Lovastatina
/
Inibidores de Hidroximetilglutaril-CoA Redutases
/
Fermentação
Tipo de estudo:
Estudos de avaliação
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
University of Mumbai/IN
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