Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization
Braz. j. microbiol
;
41(1): 179-185, Jan.-Mar. 2010. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-531750
ABSTRACT
This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Peptídeo Hidrolases
/
Inibidores de Proteases
/
Bacillus
/
Cromatografia em Gel
/
Lipase
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Asan memorial college/IN
/
Bharathi Women's College/IN
/
Vels University/IN
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