Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
Electron. j. biotechnol
;
11(3): 32-41, July 2008. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-531895
ABSTRACT
Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under-exploited tropical legume. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Among these markers, RAPD primers generated 987 amplification products of which 719 were polymorphic and ISSR markers produced 479 amplification products, out of which 296 were polymorphic. RAPD fingerprinting detected more polymorphic loci (70.30 percent) than the ISSR fingerprinting (61.79 percent). Mean PIC (polymorphism information content) for each of these marker systems (0.243 for RAPD and 0.203 for ISSR) suggested that both the marker systems were equally effective in determining polymorphisms. The dendrograms constructed using RAPD and ISSR marker systems were highly correlated with each other as revealed by high Mantel correlation (r = 0.95). Pairwise similarity index values ranged from 0.530 to 0.782 (RAPD), 0.608 and 0.862 (ISSR) and 0.559 to 0.777 (RAPD and ISSR) and mean similarity index value of 0.677, 0.729 and 0.694 for RAPD, ISSR and combined data, respectively. RAPD and ISSR marker systems were found to be useful for the genetic diversity studies in V. umbellata and identify variation within landraces.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Polimorfismo Genético
/
Análise de Sequência de DNA
/
Fabaceae
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Tamil Nadu Agricultural University/IN
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