Induction of programmed cell death by Prangos uloptera, a medicinal plant
Biol. Res
;
42(4): 517-522, 2009. graf, ilus
Artigo
em Inglês
| LILACS
| ID: lil-537111
ABSTRACT
Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50 percent inhibition concentration and 50 percent cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic blebbing. These findings were confirmed by DNA fragmentation analysis. The results demonstrated efficient induction of apoptosis by the plant extract in moderate concentrations, but administration of higher concentrations showed that the primary manner of cell death was necrosis.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Extratos Vegetais
/
Apoptose
/
Apiaceae
Limite:
Humanos
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Irã
Instituição/País de afiliação:
University of Mohaghegh Ardabili/IR
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