Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
Mem. Inst. Oswaldo Cruz
;
105(1): 109-112, Feb. 2010. tab
Artigo
em Inglês
| LILACS
| ID: lil-539304
ABSTRACT
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3 percent sensitivity, 93.3 percent specificity, a 99.2 percent positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Leishmaniose Cutânea
/
Leishmania
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fiocruz/BR
/
Instituto de Pesquisa Clínica Evandro Chagas/BR
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