Agmatine inhibits hypoxia-induced TNF-alpha release from cultured retinal ganglion cells
Biocell
;
32(2): 201-205, Aug. 2008. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-541115
ABSTRACT
The effect of hypoxia on the release of tumor necrosis factor-alpha (TNF-alpha) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-alpha in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100 microM agmatine. The expression levels of TNF-alpha and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-alpha production in RGCs. Agmatine significantly reduced TNF-alpha level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or agmatine treatment. Our results show that agmatine inhibits the TNF-alpha production of RGCs in hypoxic condition. These results demonstrate a possible neuroprotective mechanism for agmatine against hypoxic damage in RGCs.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Hipóxia Celular
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Agmatina
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Coréia do Sul
Instituição/País de afiliação:
Yonsei University College of Medicine/KR
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