Antimycobacterial susceptibility testing methods for natural products research
Braz. j. microbiol
;
41(2): 270-277, Apr.-June 2010.
Artigo
em Inglês
| LILACS
| ID: lil-545328
ABSTRACT
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis underscores the need of continuous developments on new and efficient methods to determine the susceptibility of isolates of M. tuberculosis in the search for novel antimicrobial agents. Natural products constitute an important source of new drugs, but design and implementation of antimycobacterial susceptibility testing methods are necessary for evaluate the different extracts and compounds. A number of biological assay methodologies are in current use, ranging from the classical disk diffusion and broth dilution assay format, to radiorespirometric (BACTEC), dye-based, and fluorescent/luminescence reporter assays. This review presents an analysis on the in vitro susceptibility testing methods developed for determinate antitubercular activity in natural products and related compounds (semi-synthetic natural products and natural products-derived compounds) and the criteria to select the adequate method for determination of biological activity of new natural products.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Bioensaio
/
Produtos Biológicos
/
Técnicas In Vitro
/
Sequência de Bases
/
Suscetibilidade a Doenças
/
Corantes Fluorescentes
/
Antibacterianos
/
Infecções por Mycobacterium
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Colômbia
Instituição/País de afiliação:
Instituto Nacional de Salud/CO
/
Universidad Industrial de Santander/CO
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