Characterization of 23S rRNA domain V mutations in gastric biopsy patients from the eastern Amazon
Mem. Inst. Oswaldo Cruz
;
105(3): 314-317, May 2010. tab
Artigo
em Inglês
| LILACS
| ID: lil-547302
ABSTRACT
Resistance of Helicobacter pylori to clarithromycin is characterised by simple point mutations in the 23S ribosomal RNA (rRNA) gene and is responsible for the majority of cases of failure to eradicate this bacterium. In this paper, we characterised the variability of the 23S rRNA gene in biopsies of patients with gastric pathologies in the eastern Amazon (Northern Region of Brazil) using PCR and sequencing. A total of 49 sequences of H. pylori strains were analysed and of those, 75.6 percent presented nucleotide substitutions A2142G (3.3 percent), T2182C (12.9 percent), G2224A (6.45 percent), T2215C (61.3 percent), A2192G (3.3 percent), G2204C (6.4 percent) and T2221C (6.4 percent). Of the mutations identified, four are known mutations related to cases of resistance and 16.1 percent are not yet described, revealing a high prevalence of mutations in the H. pylori 23S rRNA gene among the strains circulating in the in the eastern Amazon. The high prevalence in individuals with gastric pathologies in the Northern Region of Brazil demonstrates the need for characterising the profile of these strains to provide correct therapy for patients, considering that mutations in this gene are normally associated with resistance to the primary medication used in controlling H. pylori infection.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Gastropatias
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Mutação Puntual
/
Farmacorresistência Bacteriana
Tipo de estudo:
Fatores de risco
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Hospital Ofir Loyola/BR
/
Instituto de Ciências Biológicas/BR
/
Universidade Federal do Pará/BR
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