Adaptation of an evaluation method of 5 alpha-reductase type 1 and type 2 enzyme activities in prostate tissue
Appl. cancer res
;
29(2): 65-68, Apr.-June 2009. ilus
Artigo
em Inglês
| LILACS, Inca
| ID: lil-547667
ABSTRACT
Objectives:
We are proposing the adaptation of a method for the assessment of the activity of 5 alpha-reductase type 1 and type 2 isoenzymes in human prostate tissue to be used in studies of the relationship of enzymatic activity and cancer. Material andMethods:
We have been developing a method, based on Thomas et al., 2003 which consists of collecting human prostate samples and performing 5 alpha-reductase activity assessment. We are proposing a method based on samples obtained from prostate biopsies, according to a pilot study developed by Oliveira et al. 2006. We have obtained two samples of the same prostate area, and our idea is to send one of these samples for pathological examination and another for 5 alpha-reductase evaluation. Herein we have showed the feasibility of this assay. Through a thin layer chromatography with 14C-testosterone and NADPH (nicotinamide adenine dinucleotide phosphate) cofactor, we have obtained radioactive spots which were detected by autoradiography. The areas associated with testosterone and its metabolite DHT (dihydrotestosterone) were scrapped and counted with a scintillation chamber.Results:
We believe that it is possible to demonstrate the activity of 5 alpha-reductase through the adaptation of this biochemistry method.Conclusions:
This method is feasible and can be utilized to assess 5 alpha-reductase type 1 and type 2 activity in prostate tissue samples.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Prostatectomia
/
Neoplasias da Próstata
/
Androgênios
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Appl. cancer res
Assunto da revista:
Neoplasias
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Rio Grande do Sul/BR
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