Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae / Characterization of the variable region within class 1 integrons in Klebsiella pneumoniae nosocomials strains
Rev. méd. Chile
;
138(3): 322-329, mar. 2010. ilus, tab
Artigo
em Espanhol
| LILACS
| ID: lil-548167
ABSTRACT
Background:
Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria.Aim:
To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material andMethods:
Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation.Results:
Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found.Conclusions:
The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Infecções por Klebsiella
/
Infecção Hospitalar
/
Farmacorresistência Bacteriana Múltipla
/
Integrons
/
Klebsiella pneumoniae
/
Antibacterianos
Limite:
Humanos
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Venezuela
Instituição/País de afiliação:
Universidad Central de Venezuela/VE
/
Universidad de Oriente/VE
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