Signal to cut-off (S/CO) ratio and detection of HCV genotype 1 by real-time PCR one-step method: is there any direct relationship?
Braz. j. infect. dis
;
14(2): 147-152, Mar.-Apr. 2010. graf
Artigo
em Inglês
| LILACS
| ID: lil-548459
ABSTRACT
BACKGROUND:
Polymerase chain reaction (PCR) methods play an essential role in providing data related to diagnosis, monitoring and treatment of hepatitis C virus (HCV) infection. EIA results are reported as ''reactive'' or ''non reactive'' and EIA S/CO ratio may also be reported as ''high'' or ''low.'' This study aimed to evaluate the performance of a real-time RT-PCR and assess whether there is relationship between S/CO and PCR results. STUDY DESIGN ANDMETHODS:
Sera from blood donors were analyzed by Enzyme-Linked Immunosorbent Assay (ELISA) and RT-PCR assay to detect HCV infection.RESULTS:
The RT-PCR assay to genotypes 1a/b showed an acceptable linear response in serial dilutions. The samples were divided into two groups based on their serologicalresults:
group A - S/CO ratio < 3 (60 samples) and group B - S/CO ratio > 3 (41 samples). Viral loads were confirmed positive in group B samples in 90 percent, and in group A samples were confirmed positive in only 13 percent by RT-PCR.CONCLUSION:
The methodology used was able to detect the presence of RNA-HCV genotype I in 90 percent of the samples serologically positive in group B. All negative samples were sent to search for other genotypes of HCV (genotypes 2-6) and were confirmed as negative. These data suggests that these negative samples may have HCV RNA viral load below the detection limit of our test (310 IU/ mL), or a false positive result in serological test, or spontaneous viral clearance occurred.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
RNA Viral
/
Hepatite C
/
Hepacivirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Associação Beneficente de Coleta de Sangue/BR
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