Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa
Braz. j. microbiol
;
41(3): 588-595, Oct. 2010. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-549399
ABSTRACT
The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160) and to analyze the values according to the patient groups, infection localization and antimicrobial resistance. The following frequencies in all studied strains were established algD (encoding GDP-mannose 6-dehydrogenase AlgD) - 91.1 percent, pilB (type IV fimbrial biogenesis protein PilB) - 23.8 percent, nan1 (neuraminidase) - 21.3 percent, lasB (elastase LasB) - 100 percent, plcH (haemolytic phospholipase C precursor) - 91.6 percent, exoS (exoenzyme S) - 62.4 percent, and exoU (exoenzyme U) - 30.2 percent. The prevalence of nan1 was significantly higher (P<0.01) in CF isolates (38.1 percent) than that in non-CF isolates (16.9 percent). The nan1-positive CF strains were cultured from 16 patients with recurrent lung exacerbations. This study revealed a statistically significant difference (P<0.01) between the portion of multidrug-resistant (MDR) nosocomial P. aeruginosa strains containing a large number (¡Ý5) of virulence genes (38.1 percent) and the respective part of non-MDR isolates (17.6 percent). Moreover, pilB, exoU and nan1 manifested a higher spread (P<0.001) among MDR than in non-MDR strains (respectively, 39.1 percent vs. 13.2 percent; 40.2 percent vs. 17.7 percent and 26.1 percent vs. 4.4 percent). In conclusion, the dissemination of nan1 in CF isolates was moderate and correlated with the lower proportion of patients with lung exacerbations. The molecular-genetic detection of this gene may be used as an indirect measure of CF pulmonary disease evolution. Simultaneous determination of virulence factors and antimicrobial resistance is the contemporary approach for examination of the microbiological aspects of infections caused by P. aeruginosa.
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Índice:
LILACS (Américas)
Assunto principal:
Pseudomonas aeruginosa
/
Infecções por Pseudomonas
/
Técnicas In Vitro
/
Resistência Microbiana a Medicamentos
/
Reação em Cadeia da Polimerase
/
Fibrose Cística
/
Frequência do Gene
Tipo de estudo:
Estudos de avaliação
/
Estudo de prevalência
/
Fatores de risco
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Bulgária
Instituição/País de afiliação:
Medical University of Sofia/BG
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