Genetic and physical map of broad host range cosmid pRG930cm

Chibeu, Andrew; Lavigne, Rob; Mattheus, Wesley; Matthijs, Sandra; Cornelis, Pierre; Volckaert, Guido

Chibeu, Andrew; Lavigne, Rob; Mattheus, Wesley; Matthijs, Sandra; Cornelis, Pierre; Volckaert, Guido.

Chibeu, Andrew; Vrije Universiteit Brussel. Flanders Interuniversity Institute of Biotechnology. Department of Cellular and Molecular Interactions. Laboratory of Microbial Interactions. Brussels. BE
Lavigne, Rob; Katholieke Universiteit Leuven. Division of Gene Technology. Leuven. BE
Mattheus, Wesley; Katholieke Universiteit Leuven. Division of Gene Technology. Leuven. BE
Matthijs, Sandra; Vrije Universiteit Brussel. Flanders Interuniversity Institute of Biotechnology. Department of Cellular and Molecular Interactions. Laboratory of Microbial Interactions. Brussels. BE
Cornelis, Pierre; Vrije Universiteit Brussel. Flanders Interuniversity Institute of Biotechnology. Department of Cellular and Molecular Interactions. Laboratory of Microbial Interactions. Brussels. BE
Volckaert, Guido; Katholieke Universiteit Leuven. Division of Gene Technology. Leuven. BE

Electron. j. biotechnol ; 12(2): 10-11, Apr. 2009. ilus, tab

Artigo em Inglês | LILACS | ID: lil-551371

ABSTRACT

ABSTRACT

We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.

Assuntos

Azorhizobium caulinodans/genética; Cosmídeos; Escherichia coli/genética; Pseudomonas/genética; Agrobacterium tumefaciens/genética; Resistência ao Cloranfenicol; Engenharia Genética; Espectinomicina; Estreptomicina

Cosmid; PRG930cm; Pseudomonas; Sequencing

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Pseudomonas / Cosmídeos / Agrobacterium tumefaciens / Azorhizobium caulinodans / Escherichia coli Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2009 Tipo de documento: Artigo País de afiliação: Bélgica Instituição/País de afiliação: Katholieke Universiteit Leuven/BE / Vrije Universiteit Brussel/BE

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