S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
Braz. oral res
;
24(2): 135-141, Apr.-June 2010. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-553898
ABSTRACT
The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10 percent CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12 percent (concentration with recognized anti-plaque effect) and 0.05 percent NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05 percent NaF did not show antimicrobial effect but had similar effect to that of 0.12 percent CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fluoreto de Sódio
/
Streptococcus mutans
/
Clorexidina
/
Desmineralização do Dente
/
Biofilmes
/
Boca
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. oral res
Assunto da revista:
Odontologia
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
University of Campinas/BR
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