Glycosaminoglycan distribution in the rat uterine cervix during the estrous cycle
Clinics
;
65(7): 703-708, 2010. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-555502
ABSTRACT
OBJECTIVE:
To analyze the amount of glycosaminoglycans in the uterine cervix during each phase of the rat estrous cycle.DESIGN:
Based on vaginal smears, forty female, regularly cycling rats were divided into four groups (n = 10 for each group) GI - proestrous, GII - estrous, GIII - metaestrous and GIV - diestrous. Animals were sacrificed at each phase of the cycle, and the cervix was immediately removed and submitted to biochemical extraction and determination of sulfated glycosaminoglycans and hyaluronic acid. The results were analyzed by ANOVA followed by the Bonferroni post-hoc test.RESULTS:
The uterine cervix had the highest amount of total sulfated glycosaminoglycans and dermatan sulfate during the estrous phase (8.90 ± 0.55 mg/g of cetonic extract, p<0.001; and 8.86 ± 0.57 mg/g of cetonic extract, p<0.001). In addition, there was more heparan sulfate at the cervix during the proestrous phase (0.185 ± 0.03 mg/g of cetonic extract) than during any other phase (p<0.001). There were no significant changes in the concentration of hyaluronic acid in the uterine cervix during the estrous cycle.CONCLUSION:
Our data suggest that the amount of total sulfated glycosaminoglycans may be influenced by hormonal fluctuations related to the estrous cycle, with dermatan sulfate and heparan sulfate being the glycosaminoglycans most sensitive to hormonal change.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Colo do Útero
/
Ciclo Estral
/
Glicosaminoglicanos
Limite:
Animais
Idioma:
Inglês
Revista:
Clinics
Assunto da revista:
Medicina
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Escola Paulista de Medicina/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de São Paulo/BR
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