Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells
Clinics
;
65(7): 709-714, 2010. ilus
Artigo
em Inglês
| LILACS
| ID: lil-555503
ABSTRACT
OBJECTIVE:
Nitric oxide produced by endothelial nitric oxide synthase (eNOS) possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs).METHODS:
HUVECs were divided into four groups control, treatment with 180 ìM hydrogen peroxide (H2O2), treatment with 150 ìg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H2O2 for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction.RESULTS:
Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs.CONCLUSION:
Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Extratos Vegetais
/
Estresse Oxidativo
/
Piper
/
Óxido Nítrico Sintase Tipo III
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Inglês
Revista:
Clinics
Assunto da revista:
Medicina
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Malásia
Instituição/País de afiliação:
Universiti Kebangsaan/MY
Similares
MEDLINE
...
LILACS
LIS