In vitro regeneration and genetic fidelity of Tigridia pavonia (L. f.) DC
Electron. j. biotechnol
;
13(1): 7-8, Jan. 2010. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-559590
ABSTRACT
Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 uM 2,4-dichlorophenoxy acetic acid in combination with 2.2 uM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g I-1 activated charcoal and 2.6 uM naphtalenacetic acid. The rooted shoots achieved 100 percent survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Adenina
/
Raízes de Plantas
/
DNA de Plantas
/
Flores
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
México
Instituição/País de afiliação:
Universidad Autónoma del Estado de México/MX
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