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Characterization of an Argentine isolate of Bacillus thuringiensis similar to the HD-1 strain
Sauka, Diego H; Basurto-Ríos, Regina E; Ibarra, Jorge E; Benintende, Graciela B.
  • Sauka, Diego H; s.af
  • Basurto-Ríos, Regina E; s.af
  • Ibarra, Jorge E; s.af
  • Benintende, Graciela B; s.af
Neotrop. entomol ; 39(5): 767-773, Sept.-Oct. 2010. ilus, tab
Artigo em Inglês | LILACS | ID: lil-566216
ABSTRACT
We report the characterization of an Argentine isolate of Bacillus thuringiensis (INTA TA24-6) similar to the HD-1 strain, which harbors a cryptic cry2Ab gene that apparently is transcribed but not translated into a protein. INTA TA24-6 showed a Rep-PCR pattern identical to the HD-1 strain, a plasmid pattern that resembled that of this strain and cry1 and cry2 genes as HD-1. Screening of cry1 and cry2 genes showed that INTA TA24-6 harbors only cry1Ac and cry2Ab genes. Furthermore, crystalline inclusions of INTA TA24-6 exhibit a bipyramidal shape, typical of Lepidoptera-active B. thuringiensis strains, containing a major protein of ca. 130 kDa toxic to Epinotia aporema Wals. (Lepidoptera Tortricidae) larvae. Neither the flat-square to cuboidal crystal nor a ca. 65 kDa protein typical of strains expressing Cry2 proteins were detected in INTA TA24-6. In agreement with this information, parasporal crystals of INTA TA24-6 did not show toxicity to Aedes aegypti L. (DipteraCulicidae) larvae. Gene transcription analyses suggested that the cry2A gene might be cryptic in INTA TA24-6 despite its transcription at different sporulation stages.
Assuntos

Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Bacillus thuringiensis / Proteínas de Bactérias País/Região como assunto: América do Sul / Argentina Idioma: Inglês Revista: Neotrop. entomol Assunto da revista: Biologia / ZOOLOGIA Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Bacillus thuringiensis / Proteínas de Bactérias País/Região como assunto: América do Sul / Argentina Idioma: Inglês Revista: Neotrop. entomol Assunto da revista: Biologia / ZOOLOGIA Ano de publicação: 2010 Tipo de documento: Artigo