Cultivo y Caracterización In Vitro de una Línea Celular de Fibroblastos Gingivales Humanos / In vitro culture and characterization of a cell line of human gingival fibroblasts
Rev. Estomat
;
9(1): 27-32, jul. 2000. ilus
Artigo
em Espanhol
| LILACS
| ID: lil-569553
ABSTRACT
The main goal is to establish a cellular line of fibroblast which come from human gingival tissue, identifying its morphologic featores. Samples were taken from gum of healthy patients without medical antecedents. Three females and three male were selected. Ages were between 16 and 30 years old. A sample of 0,5 cm² of gum was obtained to take it to a cellular growth environment. Cultures were incobated at 37C, CO² atmosphere at 5% for ten (10) days. Samples were observed daily with inverted light Microscope Olimpus II. At 6th day growth of fusiform cells was observed; their morphology was in coincidence with that of the fibroblasts, At 18th day a monolayer of 100% purity was observed. The cellular line was preserved and stored by freezing in liquid nitrogen.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Linhagem Celular
/
Fibroblastos
Idioma:
Espanhol
Revista:
Rev. Estomat
Assunto da revista:
Odontologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Colômbia
Instituição/País de afiliação:
Universidad del Valle/CO
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