Human T-Lymphotropic virus (HTLV) type I in vivo integration in oral keratinocytes
Braz. j. microbiol
;
42(1): 310-320, Jan.-Mar. 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-571405
ABSTRACT
Although the infection of HTLV-1 to cell components of the mouth have been previously reported, there was not until this report, a detailed study to show the characteristics of such infection. From 14 Tropical Spastic Paraparesis/ HTLV-1-Associated Myelopathy (HAM/TSP) patients and 11 asymptomatic carrier individuals (AC) coming from HTLV-1 endemic areas of southwest Pacific of Colombia, infected oral mucosa cells were primary cultured during five days. These cell cultures were immunophenotyped by dual color fluorescence cell assortment using different lymphocyte CD markers and also were immunohistochemically processed using a polyclonal anti-keratin antibody. Five days old primary cultures were characterized as oral keratinocytes, whose phenotype was CD3- /CD4-/CD8-/CD19-/CD14-/CD45-/A575-keratin+. From DNA extracted of primary cultures LTR, pol, env and tax HTLV-1 proviral DNA regions were differentially amplified by PCR showing proviral integration. Using poly A+ RNA obtained of these primary cultures, we amplify by RT-PCR cDNA of tax and pol in 57.14 percent (8/14) HAM/TSP patients and 27.28 percent (3/11) AC. Tax and pol poly A+ RNA were expressed only in those sIgA positive subjects. Our results showed that proviral integration and viral gene expression in oral keratinocytes are associated with a HTLV-1 specific local mucosal immune response only in those HTLV-1 infected individuals with detectable levels of sIgA in their oral fluids. Altogether the results gave strong evidence that oral mucosa infection would be parte of the systemic spreading of HTLV-1 infection.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Infecções Tumorais por Vírus
/
Técnicas In Vitro
/
Imuno-Histoquímica
/
Anticorpos Antideltaretrovirus
/
Queratinócitos
/
Reação em Cadeia da Polimerase
/
Imunofenotipagem
/
HIV
/
Vírus da Reticuloendoteliose
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Colômbia
Instituição/País de afiliação:
University of Valle/CO
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