Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
Braz. j. microbiol
;
42(1): 321-329, Jan.-Mar. 2011. tab
Artigo
em Inglês
| LILACS
| ID: lil-571406
ABSTRACT
The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8 percent and 52 percent in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29 percent and 26.9 percent in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Urina
/
Sangue
/
Técnicas In Vitro
/
Reação em Cadeia da Polimerase
/
Genoma Bacteriano
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundação Oswaldo Cruz/BR
/
Universidade Federal de Pernambuco/BR
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