Tannase production by Penicillium atramentosum KM under SSF and its applications in wine clarification and tea cream solubilization
Braz. j. microbiol
;
42(1): 374-387, Jan.-Mar. 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-571412
ABSTRACT
Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar (Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was found to be the best for tannase production. Supplementation of sodium nitrate (NaNO3) as nitrogen source had enhanced tannase production both in jamun and keekar leaves. Applications of the enzyme were studied in wine clarification and tea cream solubilization. It resulted in 38.05 percent reduction of tannic acid content in case of jamun wine, 43.59 percent reduction in case of grape wine and 74 percent reduction in the tea extract after 3 h at 35ºC.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Penicillium
/
Taninos Hidrolisáveis
/
Ativação Enzimática
/
Fermentação
/
Hidrolases
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Kurukshetra University/IN
/
National Dairy Research Institute/IN
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