The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS
Braz. arch. biol. technol
;
54(1): 125-132, Jan.-Feb. 2011. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-576768
ABSTRACT
In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14 percent PEG and 13 percent salt and other at 12.6 percent PEG and 12.2 percent salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40ºC.
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Índice:
LILACS (Américas)
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Campinas/BR
/
Universidade Nove de Julho/BR
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