DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis
J. venom. anim. toxins incl. trop. dis
;
17(1): 98-102, 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-576888
ABSTRACT
This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is necessary for the diagnosis of many diseases in the future, especially infectious and parasitic ones.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Saliva
/
Leishmania braziliensis
/
DNA
/
Leishmaniose Cutânea
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
EMBRAPA Beef Cattle/BR
/
Federal University of Mato Grosso do Sul/BR
/
University of Brasilia/BR
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