Absence of occult hepatitis B among blood donors in southern Brazil
Braz. j. infect. dis
;
15(2): 159-162, Mar.-Apr. 2011. ilus
Artigo
em Inglês
| LILACS
| ID: lil-582426
ABSTRACT
BACKGROUND:
Occult hepatitis B virus (HBV) infection is characterized by the detection of HBV DNA in serum and/or in liver in the absence of detectable hepatitis B surface antigen (HBsAg). The reported prevalence of occult hepatitis B varies markedly among populations and according to the sensitivity of the HBV DNA assay. The aim of the present study was to describe the prevalence of occult hepatitis B among HCV-infected and non-infected blood donors in Porto Alegre, Southern Brazil, using a highly sensitive real time polymerase chain reaction (PCR) method.METHODOLOGY:
Between 1995 and 1997 a sample of 178 blood donors with two positive anti-HCV ELISA tests were consecutively selected as cases, and 356 anti-HCV negative donors were selected as controls. Blood donors were randomly selected from eight blood centers in Porto Alegre, Southern Brazil, representative of the whole blood donor population. Blood samples were kept at 70ºC and defrosted for the first time for the analysis of this report. Tests previously performed in the laboratory using the same real time PCR for HBV DNA had sensitivity for detecting as low as 9 copies/mL. Among 158 blood samples from HBsAg-negative blood donors, five were anti-HBc positive, 53 tested positive for anti-HCV and 105 had anti-HCV negative. The samples analysis was performed in duplicate and all blood samples tested negative for HBV DNA.CONCLUSION:
The result reflects a very low prevalence of occult hepatitis B in our setting.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doadores de Sangue
/
DNA Viral
/
Vírus da Hepatite B
/
Hepatite C
/
Hepatite B
/
Anticorpos Anti-Hepatite B
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo de prevalência
/
Fatores de risco
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
National Institute of Science and Technology for Health Technology Assessment/BR
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